Composite

Part:BBa_K1997017:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


P+R->sGFP-N->FRB->RBS->FKBP ->sGFP-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1381
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1595


Design Notes

We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced


Source

This part was synthesized by IDT

References